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pixel intensity quantitation using metamorph 6.1 software  (MetaMorph Inc)
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MetaMorph Inc pixel intensity quantitation using metamorph 6.1 software
Quantitation of fertilization-induced changes in calcium <t>green</t> <t>fluorescence.</t> Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph <t>6.1</t> software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.
Pixel Intensity Quantitation Using Metamorph 6.1 Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quantitation of fertilization-induced changes in calcium green fluorescence. Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Analysis of Signaling Pathways in Zebrafish Development by Microinjection

doi: 10.1007/978-1-59745-202-1_6

Figure Lengend Snippet: Quantitation of fertilization-induced changes in calcium green fluorescence. Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.

Article Snippet: Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software.

Techniques: Quantitation Assay, Fluorescence, Injection, Software